New Insight into HPts as Hubs in Poplar Cytokinin and Osmosensing Multistep Phosphorelays: Cytokinin Pathway Uses Specific HPts.
Identifieur interne : 000823 ( Main/Exploration ); précédent : 000822; suivant : 000824New Insight into HPts as Hubs in Poplar Cytokinin and Osmosensing Multistep Phosphorelays: Cytokinin Pathway Uses Specific HPts.
Auteurs : François Héricourt [France] ; Mélanie Larcher [France] ; Françoise Chefdor [France] ; Konstantinos Koudounas [France] ; Inês Carqueijeiro [France] ; Pamela Lemos Cruz [France] ; Vincent Courdavault [France] ; Mirai Tanigawa [Japon] ; Tatsuya Maeda [Japon] ; Christiane Depierreux [France] ; Frédéric Lamblin [France] ; Gaëlle Glévarec [France] ; Sabine Carpin [France]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2019.
Abstract
We have previously identified proteins in poplar which belong to an osmosensing (OS) signaling pathway, called a multistep phosphorelay (MSP). The MSP comprises histidine-aspartate kinases (HK), which act as membrane receptors; histidine phosphotransfer (HPt) proteins, which act as phosphorelay proteins; and response regulators (RR), some of which act as transcription factors. In this study, we identified the HK proteins homologous to the Arabidopsis cytokinin (CK) receptors, which are first partners in the poplar cytokinin MSP, and focused on specificity of these two MSPs (CK and OS), which seem to share the same pool of HPt proteins. Firstly, we isolated five CK HKs from poplar which are homologous to Arabidopsis AHK2, AHK3, and AHK4, namely, HK2, HK3a, HK3b, HK4a, HK4b. These HKs were shown to be functional kinases, as observed in a functional complementation of a yeast HK deleted strain. Moreover, one of these HKs, HK4a, was shown to have kinase activity dependent on the presence of CK. Exhaustive interaction tests between these five CK HKs and the 10 HPts characterized in poplar were performed using two-hybrid and BiFC experiments. The resulting partnership was compared to that previously identified between putative osmosensors HK1a/1b and HPt proteins. Finally, in planta coexpression analysis of genes encoding these potential partners revealed that almost all HPts are coexpressed with CK HKs in four different poplar organs. Overall, these results allowed us to unravel the common and specific partnerships existing between OS and CK MSP in Populus.
DOI: 10.3390/plants8120591
PubMed: 31835814
PubMed Central: PMC6963366
Affiliations:
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of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192</wicri:regionArea><wicri:noRegion>Shizuoka 431-3192</wicri:noRegion></affiliation></author><author><name sortKey="Maeda, Tatsuya" sort="Maeda, Tatsuya" uniqKey="Maeda T" first="Tatsuya" last="Maeda">Tatsuya Maeda</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192</wicri:regionArea><wicri:noRegion>Shizuoka 431-3192</wicri:noRegion></affiliation></author><author><name sortKey="Depierreux, Christiane" sort="Depierreux, Christiane" uniqKey="Depierreux C" first="Christiane" last="Depierreux">Christiane Depierreux</name><affiliation wicri:level="3"><nlm:affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Lamblin, Frederic" sort="Lamblin, Frederic" uniqKey="Lamblin F" first="Frédéric" last="Lamblin">Frédéric Lamblin</name><affiliation wicri:level="3"><nlm:affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Glevarec, Gaelle" sort="Glevarec, Gaelle" uniqKey="Glevarec G" first="Gaëlle" last="Glévarec">Gaëlle Glévarec</name><affiliation wicri:level="3"><nlm:affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author><author><name sortKey="Carpin, Sabine" sort="Carpin, Sabine" uniqKey="Carpin S" first="Sabine" last="Carpin">Sabine Carpin</name><affiliation wicri:level="3"><nlm:affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31835814</idno><idno type="pmid">31835814</idno><idno type="doi">10.3390/plants8120591</idno><idno type="pmc">PMC6963366</idno><idno 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45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Larcher, Melanie" sort="Larcher, Melanie" uniqKey="Larcher M" first="Mélanie" last="Larcher">Mélanie Larcher</name><affiliation wicri:level="3"><nlm:affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Chefdor, Francoise" sort="Chefdor, Francoise" uniqKey="Chefdor F" first="Françoise" last="Chefdor">Françoise Chefdor</name><affiliation wicri:level="3"><nlm:affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Koudounas, Konstantinos" sort="Koudounas, Konstantinos" uniqKey="Koudounas K" first="Konstantinos" last="Koudounas">Konstantinos Koudounas</name><affiliation wicri:level="3"><nlm:affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author><author><name sortKey="Carqueijeiro, Ines" sort="Carqueijeiro, Ines" uniqKey="Carqueijeiro I" first="Inês" last="Carqueijeiro">Inês Carqueijeiro</name><affiliation wicri:level="3"><nlm:affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author><author><name sortKey="Lemos Cruz, Pamela" sort="Lemos Cruz, Pamela" uniqKey="Lemos Cruz P" first="Pamela" last="Lemos Cruz">Pamela Lemos Cruz</name><affiliation wicri:level="3"><nlm:affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author><author><name sortKey="Courdavault, Vincent" sort="Courdavault, Vincent" uniqKey="Courdavault V" first="Vincent" last="Courdavault">Vincent Courdavault</name><affiliation wicri:level="3"><nlm:affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author><author><name sortKey="Tanigawa, Mirai" sort="Tanigawa, Mirai" uniqKey="Tanigawa M" first="Mirai" last="Tanigawa">Mirai Tanigawa</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192</wicri:regionArea><wicri:noRegion>Shizuoka 431-3192</wicri:noRegion></affiliation></author><author><name sortKey="Maeda, Tatsuya" sort="Maeda, Tatsuya" uniqKey="Maeda T" first="Tatsuya" last="Maeda">Tatsuya Maeda</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192</wicri:regionArea><wicri:noRegion>Shizuoka 431-3192</wicri:noRegion></affiliation></author><author><name sortKey="Depierreux, Christiane" sort="Depierreux, Christiane" uniqKey="Depierreux C" first="Christiane" last="Depierreux">Christiane Depierreux</name><affiliation wicri:level="3"><nlm:affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Lamblin, Frederic" sort="Lamblin, Frederic" uniqKey="Lamblin F" first="Frédéric" last="Lamblin">Frédéric Lamblin</name><affiliation wicri:level="3"><nlm:affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Glevarec, Gaelle" sort="Glevarec, Gaelle" uniqKey="Glevarec G" first="Gaëlle" last="Glévarec">Gaëlle Glévarec</name><affiliation wicri:level="3"><nlm:affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author><author><name sortKey="Carpin, Sabine" sort="Carpin, Sabine" uniqKey="Carpin S" first="Sabine" last="Carpin">Sabine Carpin</name><affiliation wicri:level="3"><nlm:affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author></analytic><series><title level="j">Plants (Basel, Switzerland)</title><idno type="ISSN">2223-7747</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have previously identified proteins in poplar which belong to an osmosensing (OS) signaling pathway, called a multistep phosphorelay (MSP). The MSP comprises histidine-aspartate kinases (HK), which act as membrane receptors; histidine phosphotransfer (HPt) proteins, which act as phosphorelay proteins; and response regulators (RR), some of which act as transcription factors. In this study, we identified the HK proteins homologous to the Arabidopsis cytokinin (CK) receptors, which are first partners in the poplar cytokinin MSP, and focused on specificity of these two MSPs (CK and OS), which seem to share the same pool of HPt proteins. Firstly, we isolated five CK HKs from poplar which are homologous to Arabidopsis AHK2, AHK3, and AHK4, namely, <i>HK2</i>, <i>HK3a</i>, <i>HK3b</i>, <i>HK4a</i>, <i>HK4b</i>. These HKs were shown to be functional kinases, as observed in a functional complementation of a yeast HK deleted strain. Moreover, one of these HKs, <i>HK4a</i>, was shown to have kinase activity dependent on the presence of CK. Exhaustive interaction tests between these five CK HKs and the 10 HPts characterized in poplar were performed using two-hybrid and BiFC experiments. The resulting partnership was compared to that previously identified between putative osmosensors <i>HK1a</i>/<i>1b</i> and HPt proteins. Finally, in planta coexpression analysis of genes encoding these potential partners revealed that almost all HPts are coexpressed with CK HKs in four different poplar organs. Overall, these results allowed us to unravel the common and specific partnerships existing between OS and CK MSP in <i>Populus</i>.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31835814</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>12</Issue><PubDate><Year>2019</Year><Month>Dec</Month><Day>11</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>New Insight into HPts as Hubs in Poplar Cytokinin and Osmosensing Multistep Phosphorelays: Cytokinin Pathway Uses Specific HPts.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E591</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants8120591</ELocationID><Abstract><AbstractText>We have previously identified proteins in poplar which belong to an osmosensing (OS) signaling pathway, called a multistep phosphorelay (MSP). The MSP comprises histidine-aspartate kinases (HK), which act as membrane receptors; histidine phosphotransfer (HPt) proteins, which act as phosphorelay proteins; and response regulators (RR), some of which act as transcription factors. In this study, we identified the HK proteins homologous to the Arabidopsis cytokinin (CK) receptors, which are first partners in the poplar cytokinin MSP, and focused on specificity of these two MSPs (CK and OS), which seem to share the same pool of HPt proteins. Firstly, we isolated five CK HKs from poplar which are homologous to Arabidopsis AHK2, AHK3, and AHK4, namely, <i>HK2</i>, <i>HK3a</i>, <i>HK3b</i>, <i>HK4a</i>, <i>HK4b</i>. These HKs were shown to be functional kinases, as observed in a functional complementation of a yeast HK deleted strain. Moreover, one of these HKs, <i>HK4a</i>, was shown to have kinase activity dependent on the presence of CK. Exhaustive interaction tests between these five CK HKs and the 10 HPts characterized in poplar were performed using two-hybrid and BiFC experiments. The resulting partnership was compared to that previously identified between putative osmosensors <i>HK1a</i>/<i>1b</i> and HPt proteins. Finally, in planta coexpression analysis of genes encoding these potential partners revealed that almost all HPts are coexpressed with CK HKs in four different poplar organs. Overall, these results allowed us to unravel the common and specific partnerships existing between OS and CK MSP in <i>Populus</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Héricourt</LastName><ForeName>François</ForeName><Initials>F</Initials><Identifier Source="ORCID">0000-0002-6463-3697</Identifier><AffiliationInfo><Affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Larcher</LastName><ForeName>Mélanie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chefdor</LastName><ForeName>Françoise</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koudounas</LastName><ForeName>Konstantinos</ForeName><Initials>K</Initials><Identifier Source="ORCID">0000-0002-6000-6565</Identifier><AffiliationInfo><Affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carqueijeiro</LastName><ForeName>Inês</ForeName><Initials>I</Initials><Identifier Source="ORCID">0000-0001-9576-5556</Identifier><AffiliationInfo><Affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lemos Cruz</LastName><ForeName>Pamela</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Courdavault</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><Identifier Source="ORCID">0000-0001-8902-4532</Identifier><AffiliationInfo><Affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tanigawa</LastName><ForeName>Mirai</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maeda</LastName><ForeName>Tatsuya</ForeName><Initials>T</Initials><Identifier Source="ORCID">0000-0001-9952-1612</Identifier><AffiliationInfo><Affiliation>Department of Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Depierreux</LastName><ForeName>Christiane</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lamblin</LastName><ForeName>Frédéric</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Glévarec</LastName><ForeName>Gaëlle</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>BBV, University of Tours, EA 2106, 31 Avenue Monge, 37200 Tours, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carpin</LastName><ForeName>Sabine</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>LBLGC, University of Orléans, EA1207, INRA, USC1328, rue de Chartres, CEDEX 2, 45067 Orléans, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>SiSCyLi 2013-00085478</GrantID><Agency>Région Centre-Val de Loire</Agency><Country></Country></Grant><Grant><GrantID>SiSeP 2008-0033673</GrantID><Agency>Région Centre-Val de Loire</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>12</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CK</Keyword><Keyword MajorTopicYN="N">HK</Keyword><Keyword MajorTopicYN="N">HPt</Keyword><Keyword MajorTopicYN="N">MSP</Keyword><Keyword MajorTopicYN="N">OS</Keyword><Keyword MajorTopicYN="N">cytokinin</Keyword><Keyword MajorTopicYN="N">histidine phosphotransfer protein</Keyword><Keyword MajorTopicYN="N">histidine-aspartate kinase</Keyword><Keyword MajorTopicYN="N">multistep phosphorelay</Keyword><Keyword MajorTopicYN="N">osmosensing</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>12</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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sortKey="Carpin, Sabine" sort="Carpin, Sabine" uniqKey="Carpin S" first="Sabine" last="Carpin">Sabine Carpin</name><name sortKey="Carqueijeiro, Ines" sort="Carqueijeiro, Ines" uniqKey="Carqueijeiro I" first="Inês" last="Carqueijeiro">Inês Carqueijeiro</name><name sortKey="Chefdor, Francoise" sort="Chefdor, Francoise" uniqKey="Chefdor F" first="Françoise" last="Chefdor">Françoise Chefdor</name><name sortKey="Courdavault, Vincent" sort="Courdavault, Vincent" uniqKey="Courdavault V" first="Vincent" last="Courdavault">Vincent Courdavault</name><name sortKey="Depierreux, Christiane" sort="Depierreux, Christiane" uniqKey="Depierreux C" first="Christiane" last="Depierreux">Christiane Depierreux</name><name sortKey="Glevarec, Gaelle" sort="Glevarec, Gaelle" uniqKey="Glevarec G" first="Gaëlle" last="Glévarec">Gaëlle Glévarec</name><name sortKey="Koudounas, Konstantinos" sort="Koudounas, Konstantinos" uniqKey="Koudounas K" first="Konstantinos" last="Koudounas">Konstantinos Koudounas</name><name sortKey="Lamblin, Frederic" sort="Lamblin, Frederic" uniqKey="Lamblin F" first="Frédéric" last="Lamblin">Frédéric Lamblin</name><name sortKey="Larcher, Melanie" sort="Larcher, Melanie" uniqKey="Larcher M" first="Mélanie" last="Larcher">Mélanie Larcher</name><name sortKey="Lemos Cruz, Pamela" sort="Lemos Cruz, Pamela" uniqKey="Lemos Cruz P" first="Pamela" last="Lemos Cruz">Pamela Lemos Cruz</name></country><country name="Japon"><noRegion><name sortKey="Tanigawa, Mirai" sort="Tanigawa, Mirai" uniqKey="Tanigawa M" first="Mirai" last="Tanigawa">Mirai Tanigawa</name></noRegion><name sortKey="Maeda, Tatsuya" sort="Maeda, Tatsuya" uniqKey="Maeda T" first="Tatsuya" last="Maeda">Tatsuya Maeda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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